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normal human fetal osteoblast cell line  (ATCC)


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    ATCC normal human fetal osteoblast cell line
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Normal Human Fetal Osteoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+fetal+osteoblast+cell+line/TIBx%3B+Epithelial+liver%3B+Mouse/pmc12730730-62-0-8
    Average 97 stars, based on 2806 article reviews
    normal human fetal osteoblast cell line - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development"

    Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

    Journal: Biomedicines

    doi: 10.3390/biomedicines13123039

    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Figure Legend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Techniques Used: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence

    Related Articles

    Cell Culture:

    Article Title: Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential
    Article Snippet: .. Cell culture experiments were performed using human normal fetal osteoblast cell line (hFOB 1.19, ATCC). .. The cells were cultured in a 1:1 mixture of DMEM/Ham’s F12 medium (Sigma-Aldrich, Germany) with the following supplementation: 10% fetal bovine serum (FBS, Pan-Biotech), 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 0.3 mg/mL G418 (all antibiotics were purchased from Sigma-Aldrich Chemicals).



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    normal human fetal osteoblast cell line  (ATCC)
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    ATCC normal human fetal osteoblast cell line
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Normal Human Fetal Osteoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+fetal+osteoblast+cell+line/TIBx%3B+Epithelial+liver%3B+Mouse/pmc12730730-62-0-8
    Average 97 stars, based on 1 article reviews
    normal human fetal osteoblast cell line - by Bioz Stars, 2026-09
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    Osteogenic differentiation markers assessed by ELISAs for <t>osteoblasts</t> cultured on the biomaterials and polystyrene well as a control: ( a ) type I collagen, ( b ) bone alkaline phosphatase, ( c ) osteocalcin. The level of osteogenic markers was normalized to 1 mg of cellular proteins (assessed by BCA assay). (*, **, *** statistically significant results according to unpaird Student’s t -test).
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    human normal fetal osteoblastic cell line hfob1 19 hfob  (ATCC)
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    ATCC human normal fetal osteoblastic cell line hfob1 19 hfob
    Osteogenic differentiation markers assessed by ELISAs for <t>osteoblasts</t> cultured on the biomaterials and polystyrene well as a control: ( a ) type I collagen, ( b ) bone alkaline phosphatase, ( c ) osteocalcin. The level of osteogenic markers was normalized to 1 mg of cellular proteins (assessed by BCA assay). (*, **, *** statistically significant results according to unpaird Student’s t -test).
    Human Normal Fetal Osteoblastic Cell Line Hfob1 19 Hfob, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Journal: Biomedicines

    Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

    doi: 10.3390/biomedicines13123039

    Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

    Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence

    Osteogenic differentiation markers assessed by ELISAs for osteoblasts cultured on the biomaterials and polystyrene well as a control: ( a ) type I collagen, ( b ) bone alkaline phosphatase, ( c ) osteocalcin. The level of osteogenic markers was normalized to 1 mg of cellular proteins (assessed by BCA assay). (*, **, *** statistically significant results according to unpaird Student’s t -test).

    Journal: International Journal of Molecular Sciences

    Article Title: Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential

    doi: 10.3390/ijms221910414

    Figure Lengend Snippet: Osteogenic differentiation markers assessed by ELISAs for osteoblasts cultured on the biomaterials and polystyrene well as a control: ( a ) type I collagen, ( b ) bone alkaline phosphatase, ( c ) osteocalcin. The level of osteogenic markers was normalized to 1 mg of cellular proteins (assessed by BCA assay). (*, **, *** statistically significant results according to unpaird Student’s t -test).

    Article Snippet: Cell culture experiments were performed using human normal fetal osteoblast cell line (hFOB 1.19, ATCC).

    Techniques: Cell Culture, Control, BIA-KA